| Component | Amount per reaction (μl) | Final |
|---|---|---|
| DNA library from Step 55 | Variable | |
| 1 mg/ml COT human DNA (from Roche NimbleGen kit) | 5 | 5 μg |
| P5 adapter (100 μM) | 10 | |
| Pooled barcoded P7 adapter (100 μM) | 10 | |
| Final volume | Variable |
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