| Component | Amount per reaction (μl) | Final |
|---|---|---|
| Captured sample with beads from Step 91 | 50 | |
| Adapter PCR primers, 20 μM | 6 | 0.6 μM |
| Sterile water | 44 | |
| KAPA 2× HiFi HotStart mix | 100 | 1× |
| Final volume | 200 |
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