| Component | Amount per reaction |
Final | |
|---|---|---|---|
| Sample (μl) | Standard (μl) | ||
| Genomic DNA from Step 13 | 8 | - | 5 ng |
| Genomic standards (20 or 2 nM, or 0.2 or 0.02 nM) | - | 1 | |
| Water | - | 7 | |
| Forward, 5 nM and reverse primer mix | 2 | 2 | 0.5 nM |
| KAPA SYBR Fast qPCR master mix, 2× | 10 | 10 | |
| Final volume | 20 | 20 | |
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