| Component | Amount per reaction (μl) | Final |
|---|---|---|
| DNA sample from Step 37 | 25 | |
| Annealed 10 μM barcoded adapters | 2 | 0.4 μM |
| Sterile water | 8 | |
| Quick T4 DNA ligase (from NEBNext quick ligation module) | 5 | |
| Quick ligation reaction buffer, 5× (from NEBNext quick ligation module) | 10 | 1× |
| Final volume | 50 |
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