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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Nat Protoc. 2016 Jan 7;11(2):214–235. doi: 10.1038/nprot.2016.005
Cycle number Denature Anneal Extend Hold
1 98 °C, 30 s
2-9 98 °C, 10 s 65 °C, 30 s 72 °C, 30 s
10 72 °C, 5 min
11 4 °C