Table 2.
TIMP consensus sequences, with various terminal groups and consensus thresholds, using the final, selected terminal set of 226 echinoderm and outgroup TIMPs. Location in the 833-position alignment is shown in the top row. Conserved positions shown are all cysteine residues, the VIRAK motif identified earlier in vertebrates (positions 174–178), and any conserved residues on the 70% consensus of all TIMPs.
| terminals | threshold (%) | 150 | 153 | 162 | 164 | 165 | 169 | 174 | 175 | 176 | 177 | 178 | 320 | 334 | 335 | 362 | 372 | 375 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| chordates | 70 | C | C | H | P | Q | C | V | I | R | A | K | Y | K | G | T | C | G |
| protostomes | 70 | C | C | H | P | Q | C | V | — | — | — | — | Y | K | — | T | C | G |
| Asteroidea | 70 | — | C | H | — | Q | C | V | — | — | — | — | Y | K | G | — | C | G |
| Crinoidea | 70 | — | C | H | P | Q | C | — | I | R | — | — | Y | K | G | T | C | G |
| Echinoidea | 70 | — | — | — | — | — | — | — | — | — | — | — | Y | K | — | — | C | G |
| Holothuroidea | 70 | C | C | H | P | Q | C | — | — | — | — | — | — | K | — | T | C | G |
| Ophiuroidea | 70 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | C | G |
| all | 90 | — | — | — | — | — | — | — | — | — | — | — | — | — | — | — | C | — |
| all | 80 | — | C | — | — | — | C | — | — | — | — | — | — | K | — | — | C | G |
| all | 70 | C | C | H | P | Q | C | — | — | — | — | — | Y | K | G | T | C | G |
| all | 60 | C | C | H | P | Q | C | V | — | — | — | — | Y | K | G | T | C | G |
| all | 50 | C | C | H | P | Q | C | V | I | — | — | — | Y | K | G | T | C | G |
| terminals | threshold (%) | 380 | 396 | 400 | 440 | 456 | 459 | 475 | 479 | 484 | 493 | 498 | 548 | 572 | 579 | 594 | 596 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| chordates | 70 | — | Y | G | C | Q | — | Y | C | C | C | C | C | — | C | C | W | |
| protostomes | 70 | — | Y | G | C | Q | G | Y | C | C | C | C | C | C | C | C | W | |
| Asteroidea | 70 | L | Y | G | C | Q | G | Y | C | C | — | — | C | C | C | C | W | |
| Crinoidea | 70 | L | Y | G | C | Q | G | Y | C | C | C | — | C | C | C | C | W | |
| Echinoidea | 70 | L | Y | G | C | — | — | — | — | — | — | — | — | — | — | — | — | |
| Holothuroidea | 70 | — | Y | G | C | Q | — | — | C | C | — | — | C | C | C | C | W | |
| Ophiuroidea | 70 | — | Y | — | C | — | — | Y | — | — | — | — | — | — | — | — | — | |
| all | 90 | — | — | — | C | — | — | — | C | — | — | — | — | — | — | — | — | |
| all | 80 | — | Y | G | C | Q | — | Y | C | C | — | — | C | — | — | C | W | |
| all | 70 | L | Y | G | C | Q | G | Y | C | C | — | — | C | — | C | C | W | |
| all | 60 | L | Y | G | C | Q | G | Y | C | C | — | — | C | C | C | C | W | |
| all | 50 | L | Y | G | C | Q | G | Y | C | C | C | — | C | C | C | C | W |