FIG 4.

EhBAR (EHI_105240) and EhILWEQ (EHI_003930) localization via SNAP tag labeling. Entamoeba trophozoites expressing EhBAR- and EhILWEQ-SNAP fusion proteins were labeled and fixed following a 60-min incubation on an HCT-8 host cell monolayer. EhBAR and EhILWEQ show enrichment at the site of partially formed phagosomes (yellow arrows), whereas SNAP with an ER localization tag does not. EhBAR and EhILWEQ also show enrichment in extending pseudopodia (green arrows). HCT-8 cell nuclei were stained using cell-permeant Hoechst 33342 (blue), and SNAP fusion proteins were labeled using a cell-permeant TMR-Star substrate (red). Bars = 10 μm.