Survival and bacterial loads. (A) Survival at 0 to 17 days after S. aureus inoculation after 8 weeks on HFD-S, HFD-ω3, or HFD-ω6. The log rank test was used to determine statistically significant differences (n = 20 mice per group). (B) Bacterial loads in the kidneys at day 6 after S. aureus inoculation after 8 weeks on LFD, HFD-S, HFD-ω3, or HFD-ω6. Two-way ANOVA with experiment as a nuisance factor was used to determine statistically significant differences (n = 18, 16, 18, and 10 mice per group, respectively). The data shown are the estimated marginal mean plus SEM. *, P < 0.05; ***, P < 0.001. Experimental groups: LFD, HFD-S, HFD-ω3, and HFD-ω6.