FIG 5.

Role of TLR2 and TLR4 in OMV-mediated abrogation of the TNF response. (A to D) Human monocytes were prestimulated with OMV or not prestimulated; live P. gingivalis was used for secondary challenge after washing. The experiments were performed in the presence of neutralizing anti-TLR2 (A and B) and anti-TLR4 (C and D) antibodies or of the respective isotype controls. TNF secretion was measured in the supernatants harvested after 24 (A and C) and 48 h (B and D). The diagrams summarize the data obtained as mean values ± SD of the results from n = 4 donors (n = 4 independent experiments). *, P = 0.0154 (A); n.s. = not significant (B); *, P = 0.0281; *p_§ = 0.0452; **, P = 0.0032 (C); *, P = 0.0261 (D).