FIG 2.
Measurement of caspase-1-dependent proinflammatory cytokines in supernatants of BMDMs infected with Y. pseudotuberculosis. BMDMs from C57BL/6 mice were primed with 100 ng/ml LPS for 18 h and left uninfected or infected with the 32777, ΔyopM, yopJC172A, or yopJC172A ΔyopM strain at an MOI of 30. At 90 min postinfection, supernatants were collected, and concentrations of secreted IL-1β, IL-18, or IL-1α were measured by ELISA. Data represent average values ± the standard errors of the means from three independent experiments. ***, P < 0.001; ****, P < 0.0001, as determined by one-way analysis of variance compared to values for 32777 ΔyopM-infected macrophages.