FIG 5.

Measurement of processed caspase-1, pro-IL-1β, mature IL-1β, and pyroptosis in BMDMs infected with Y. pseudotuberculosis or Y. pestis. LPS-primed BMDMs were left uninfected or infected with 32777, 32777 ΔyopM, KIM5, or KIM5 ΔyopM for 90 min at an MOI of 30. (A) After infection, lysates were collected, processed, and subjected to Western blotting using antibodies to pro-IL-1β, cleaved caspase-1, or β-actin. Supernatants were collected and analyzed for secreted IL-1β (B) or pyroptosis by measurement of LDH release (C). Data represent average values ± the standard errors of the means from three independent experiments. ****, P < 0.0001, as determined by one-way analysis of variance comparing values in BMDMs infected with 32777 ΔyopM versus those with 32777 infection or BMDMs infected with KIM5 ΔyopM versus those infected with KIM5.