TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and characteristics | Source or reference |
|---|---|---|
| 1711-4 | aEPEC O51:H40, wild type | 23 |
| E2348/69 | Typical EPEC, serotype O127:H7 | 42 |
| EDL933 | EHEC, serotype O157:H7 | 43 |
| H10407 | ETEC, serotype O78:H11 | 44 |
| 17ΔfliD | fliD::ble (Zeor) | This study |
| 17ΔmotAB | motAB::ble (Zeor) | This study |
| 17ΔfliC | fliC::ble (Zeor) | 22 |
| pET200fliD | fliD gene from 1711-4 cloned into pET200/D-TOPO | This study |
| pBADmotAB | motA motB genes from 1711-4 cloned into pET200/D-TOPO | This study |
| pZEKmfliC | fliC gene from 1711-4 cloned into pZE21-MCS2 | 22 |
| pZE21-MCS2 | Plasmid encoding Km resistance (Kmr) | 24 |
| 17(pETfliD) | 17ΔfliD carrying pET200fliD (Zeor Kmr) | This study |
| 17(pBADmotAB) | 17ΔmotAB carrying pBAD202/D-TOPO motAB (Zeor Kmr) | This study |
| C1P1-8 | 17ΔfliC carrying pZEKmfliC (Zeor Kmr) | 22 |
| MC4160-malTΔ224::zeo-(F+) | Source of Zeo cassette (ble gene) | Gift from J. M. Ghigo |
| pKOBEG-Aprar | Plasmid encoding red λ phage operon, Apra resistance (Aprar) | 45 |