TABLE 2.

Primers used for PCR amplification or sequencing

Primer	Nucleotide sequence	Amplicon size (bp)
motAB-FW	5′ GCTTATATCCATGCTCACGCTG 3′	1,800
motAB-RV	5′ GGTCAACAGTGGAAGGATGAT 3′
motAB.zeo.RV	5′ CTGAACATCCTGTCATGGTCAACAGTGGAAGGATGATGTCGTCATCGCTTGCATTAGAAAGG 3′	642
motAB.zeo.FW	5′ AAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGGAATGATGCAGAGATGTAAG 3′
pBAD-202-motAB-FW	5′ CACCTGAGTGCTTATCTTATTAGGTTAC 3′	1,953
pBAD-202-motAB-RV-1953	5′ AGCCCGAAAGATGGCATTCA 3′
fliCH40.zeo-5	5′ GCCCAATAACATCAAGTTGTAATTGATAAGGAAAAGATCATGGTCATCGCTTGCATTAGAAAG 3′	642
fliCH40.zeo-3	5′ AACCCCGCCGGTGGCGGGGTTTGAGCGATAAGTGTAAATTAGAATGATGCAGAGATGTAAG 3′
PrfliCH40F	5′ AAATTTCTCGAGACCTAATTCCTTTTGATTGCAAAC 3′	1,833
fliCH40R	5′ AAATTTTCTAGACAGGGGTTATTTGGGGGTTA 3′
Pet fliD FW	5′ CACCATGGCAAGTATTTCATGC 3′	1,450
fliD-ext-3L	5′ TTACTTGGAATTACTGTTGTTTTCG 3′
fliD.zeo.5	5′ TATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGTCATCGCTTGCATTAGAAAGG 3′	642
fliD.zeo.3	5′ TTGCCGCGTACATGACCTGTCTCCCGATGAATATTGATTAGAATGATGCAGAGATGTAAG 3′
fliD-FW-pET-Topo	5′ CACCATGGCAAGTATTTCATCG 3′	1,600a
fliD-RV-pET-Topo	5′ TCGCTTGGAATTACTGTTGTTTT 3′	200b
fliD.verifF-5′	5′ CGTCAACCCTGTTATCGTCTG 3′	1,765
fliD.verifR-3′	5′ AAACAGGCTCGCTCTAACCA 3′
pBADverf FW	5′ ATGCCAATAGCATTTTTATCC 3′	685b
pBADverf RV	5′ TCTGATTTAATCTGTATCAGG 3′	2,496a
TrxFus-FWc	5′ TTCCTCGACGCTAACCTG 3′	238b
pBAD-RVc	5′ GATTTAATCTGTATCAGG 3′	2,049a

^aWith insert.

^bWithout insert.

^cSequencing primer.