FIG 5.

Exogenous expression of slyA upregulates VirB-dependent promoters under repressed conditions. (A) Upregulation of mxiC-lacZ. β-Galactosidase activity of mxiC-lacZ cells harboring pBAD, pBAD-virB, or pBAD-slyA in derepressed (37°C) or repressed (30°C) conditions. (B) Upregulation of PicsP-lacZ. β-Galactosidase activity of cells containing PicsP-lacZ and harboring pBAD, pBAD-virB, or pBAD-slyA in derepressed (wild-type) or repressed (virB::Tn5) conditions is shown. Data are representative of three independent trials conducted in triplicate. A two-tailed Student's t test (assuming equal variance) was used for statistical analysis. *, P < 0.01; **, P < 0.001.