Skip to main content
. 2016 Mar 24;84(4):998–1015. doi: 10.1128/IAI.01536-15

FIG 3.

FIG 3

C. burnetii Nine Mile II T4SS activity does not enhance cytokine production or early p38 MAPK phosphorylation in C57BL/6 macrophages. (A and B) C57BL/6 BMDMs were infected with the L. pneumophila (Lp) ΔflaA or ΔdotA mutant at an MOI of 5 or with WT C. burnetii (Cb) or the icmL::Tn mutant NMII at an MOI of 5 or 50 for 24 h. Levels of TNF (A) and IL-6 (B) in the supernatants were measured by ELISA. (C and D) C57BL/6 and TLR2−/− BMDMs were infected with WT C. burnetii (Cb) or the icmL::Tn NMII mutant at an MOI of 50 for 24 h. Levels of TNF (C) and IL-6 (D) in the supernatants were measured by ELISA. Graphs show the means ± SEM from triplicate wells. Results are representative of three (A and B) or two (C and D) independent experiments. (E) Immunoblot analysis of phosphorylated and total p38 MAPK in cell lysates from C57BL/6 and Myd88−/− BMDMs that were mock infected or infected with WT C. burnetii NMII at an MOI of 50 for 0, 15, 30, or 120 min. Results are representative of two independent experiments.