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. 2016 Mar 24;84(4):883–893. doi: 10.1128/IAI.01361-15

TABLE 1.

Primer sequences used to quantify mRNA of genes of interest

Gene Orientationa Sequence (5′−3′) Reference(s)
β-Actin F GAT TAC TGC TCT GGC TCC TAG C 20, 21
R GAC TCA TCG TAC TCC TGC TTG C
IL-6 F CAC AAG TCC GGA GAG GAG AC 20, 21
R CAG AAT TGC CAT TGC ACA AC
IFN-γ F GTT ACT GCC ACG GCA CAG TCA TTG 20
R ACC ATC CTT TTG CCA GTT CCT CCA G
IL-10 F ACC AAC ATC CTG GTG TCT CC 22
R CAT GTC AAA CGT GAG CGA CT
TNF-α F GCA AGC TTC GCT CTT CTG TCT ACT GAA CTT CGG 20
R GCT CTA GAA TGA GAT AGC AAA TCG GCT GAC GG
IL-1β F CGC AGC AGC ACA TCA ACA AGA GC 20, 21
R TGT CCT CAT CCT GGA AGG TCC ACG
a

F, forward; R, reverse.