FIG 1.

Localization of Gal3 in the mouse stomach. (A) Immunohistochemistry (IHC) for Gal3. IHC was performed for Gal3 using tissue sections from formalin-fixed and paraffin-embedded gastrointestinal tracts obtained from WT and Gal3-deficient mice. Representative results from four different anatomical locations are shown (n = 7). (B) PAS staining. Tissue sections from formalin-fixed and paraffin-embedded gastric tissues were stained with PAS. Representative results are shown (n = 7). (C) Immunoblot analysis for Gal3. Lysates were prepared from gastric tissues and surface mucus layer samples obtained from WT and Gal3-deficient mice. Immunoblot analysis was performed for Gal3. Representative results from three separate experiments are shown.