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Effects of acetic acid on the expression of Whi2. Yeast strains with GFP fusion proteins in exponential phase of growth were incubated in SCD medium amended with acetic acid (2 g/liter or 3 g/liter [pH 4.0]) for 6 h. The protein expression level of Pgk1 was used as the reference for normalization. The normalized protein level in cells without acetic acid stress was taken as 100%. The results are the means from triplicate experiments; error bars represent standard deviations.
