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. 2016 Mar 21;82(7):2003–2011. doi: 10.1128/AEM.03445-15

TABLE 1.

Test matrixa

Test no. Surrogate microorganism(s) Target fumigation parameter value
Contact time (h)
MeBr concn (mg/liter) Temp (°C) RH (%)
1 G. stearothermophilus 212 22 45 36
2 G. stearothermophilus 212 22 45 48
3 B. anthracis NNR1Δ1 212 22 75 36
4 B. anthracis NNR1Δ1 212 27 45 36
5 B. anthracis NNR1Δ1 212 22 75 24
6 B. anthracis Sterne 212 27 45 48
7 B. anthracis Sterne 212 27 75 24
8 B. anthracis Sterne 212 27 45 48
9 B. anthracis NNR1Δ1, B. anthracis Sterne 212 27 75 36
10 B. anthracis NNR1Δ1, B. anthracis Sterne 300 22 45 48
11 B. anthracis NNR1Δ1, B. anthracis Sterne 212 22 45 60
12 B. anthracis NNR1Δ1, B. anthracis Sterne 212 32 75 24
13 B. anthracis NNR1Δ1, B. anthracis Sterne 212 32 45 48
14 B. anthracis NNR1Δ1, B. anthracis Sterne 300 22 75 24
15 B. anthracis NNR1Δ1, B. anthracis Sterne 300 22 45 60
16 B. anthracis NNR1Δ1, B. anthracis Sterne 212 32 45 60
17 B. anthracis NNR1Δ1, B. anthracis Sterne 300 27 75 18
18 B. anthracis NNR1Δ1, B. anthracis Sterne 300 27 45 60
19 B. anthracis NNR1Δ1, B. anthracis Sterne 212 32 45 72
20 B. anthracis NNR1Δ1, B. anthracis Sterne 300 32 45 60
a

All tests were conducted with B. anthracis Ames and at least one surrogate. Tests 1 to 8 were conducted with glass, ceiling tile, carpet, painted wallboard paper, bare pine wood, and unpainted concrete. Tests 9 to 20 were conducted with glass, ceiling tile, carpet, and bare pine wood.