FIG 5.
Relative activity at various temperatures of BglU and its mutants (A), the Tm value from the derivative of the fluorescent signal (B), and the fluorescence adsorption of BglU, BglB, and their mutants (C). The calculated Tm values were 36.5°C for BglU, 43.5°C for H299Y, 34°C for delta-L3/H299Y, 47°C for BglB, and 40.5°C for BglB-Y301H. BglU and its mutant enzymes were maintained at various temperatures as shown for 40 min. For each protein, activity at 0 min was defined as 100%. The Tm was determined by differential scanning fluorimetry (DSF). The experiments were performed in triplicate or quadruplicate.