TABLE 1.

Primers used for mutations

Mutation and primer	Sequence (5′-3′)a
delta-L3
P1	CGCGGATCCATGATGAACCACTTATC
P2	GCCGGGAAGGTCACATACTCGCTGCCGACAAACTGGCCGG CGGGCAGCGGAT
P3	ACGTTTCAGGCCATCCGCTGCCCGCCGGCCAGTTTGTCGGCAGCGAGTATGT
P4	CCCAAGCTTTTATGCCATTGTACGG
H299Y
Pf	CTTGGGCGTGAACTACTACCACGATGACAACG
Pr	CGTTGTCATCGTGGTAGTAGTTCACGCCCAAG
delta-L3/H229Y	All of the above

^aRestriction sites are indicated by underlining.