TABLE 3.
Kinetic parameters for BglU and its mutantsa
| Enzyme or mutant | Mean ± SEM |
|||
|---|---|---|---|---|
| Km (mmol/liter) | Vmax × 104 (μmol min−1 mg−1) | kcat × 103 (s−1) | kcat/Km × 106 (liter mol−1 s−1) | |
| BglU | 5.9 ± 0.57 | 0.843 ± 0.1 | 6.7 ± 0.7 | 1.14 ± 0.13 |
| H229Y | 3.99 ± 0.14 | 2.83 ± 0.04 | 22.64 ± 0.07 | 5.7 ± 1.00 |
| delta-L3 | 19.39 ± 1.27 | 0.058 ± 0.01 | 0.46 ± 0.09 | 0.0238 ± 0.002 |
| delta-L3/H299Y | 5.2 ± 0.6 | 0.909 ± 0.19 | 7.27 ± 1.5 | 1.48 ± 0.19 |
a
Kinetic parameters were determined using pNPG as the substrate at 25°C. The experiments were performed in triplicate.