TABLE 1.

Purification of l-Pip-hydroxylating enzyme from F. oxysporum c8D

Step	Total activity (U)	Total protein (mg)	Sp act (U/mg)	Yield (%)
Cell lysate	1.26	45	0.028	100
Toyopearl CM-650M	0.50	9.1	0.055	40
Toyopearl DEAE-650M	0.33	5.1	0.065	26
Mono Q	0.30	1.9	0.16	24
Phenyl Superose	0.067	0.32	0.21	5.3
Superdex 200	0.0022	0.0030	0.74	0.18