TABLE 3.
Substrate specificity analysis and kinetic parameters of FoPip4H and AnPip4Hb
| Substrate |
FoPip4H |
AnPip4H |
||
|---|---|---|---|---|
| Sp act (U/mg) | Km (mM) | Sp act (U/mg) | Km (mM) | |
| l-Pip | 1.6 ± 0.1 | 1.5 ± 0.1 | 0.76 ± 0.01 | 1.4 ± 0.0 |
| d-Pip | ND | 0.18 ± 0.01a | ||
| l-Pro | 0.55 ± 0.03 | 3.1 ± 0.2 | 0.37 ± 0.02 | 1.4 ± 0.1 |
| d-Pro | ND | 0.044 ± 0.008 | 3.8 ± 0.7 | |
| l-Leu | tr | ND | ||
| l-AABA | tr | ND | ||
| cis-5-l-HyPip | 0.50 ± 0.05a | 0.28 ± 0.05a | ||
a
Enzyme activities were analyzed by succinate analysis.
b
All measurements were performed at least three times. ND, not detected; tr, trace activity detected only in the amino acid analysis.