TABLE 2.
Primers used in this study
| Primer | Sequencea | Application |
|---|---|---|
| A12-arcR-5′-F | 5′-TTTCACACCCGAGCTCTTAC-3′ | A12 ΔarcR; Emr |
| A12-arcR-5′-R-SOE | 5′-ACTCCTTCTGGTGGCGACTTTCAATCTTATTC-3′ | A12 ΔarcR; Emr |
| A12-arcR-Erm-F-SOE | 5′-GTCGCCACCAGAAGGAGTGATTACATGAACAAA-3′ | A12 ΔarcR; Emr |
| A12-arcR-Erm-R-SOE | 5′-GAGGCAGACCCCTTTAGTAACGTGTAACTTT-3′ | A12 ΔarcR; Emr |
| A12-arcR-3′-F-SOE | 5′-ACTAAAGGGGTCTGCCTCATCATCTGTGAA-3′ | A12 ΔarcR; Emr |
| A12-arcR-3′-R | 5′-CTCTCCATTGATGAACAGCAA-3′ | A12 ΔarcR; Emr |
| A12-spxB-5′-F | 5′-GAATTGGCGCAAGGGATGATAA-3′ | A12 ΔspxB; Kmr |
| A12-spxB-5′-R-SOE | 5′-TTTCTACAGGATAGTGTCTACGCCCCATGTT-3′ | A12 ΔspxB; Kmr |
| A12-spxB-Kan-F-SOE | 5′-GACACTATCCTGTAGAAAAGAGGAAGGAA-3′ | A12 ΔspxB; Kmr |
| A12-spxB-Kan-R-SOE | 5′-TGGTACAAGGAGTATGGACAGTTGCGGATGTA-3′ | A12 ΔspxB; Kmr |
| A12-spxB-3′-F-SOE | 5′-TCCATACTCCTTGTACCATTCCGTCTCTTC TT-3′ | A12 ΔspxB; Kmr |
| A12-spxB-3′-R | 5′-CCACTTACTAGCAGGGATTCGGTT-3′ | A12 ΔspxB; Kmr |
| A12-sgc-5′-F | 5′-CCATTTGGCCAAGGAGAGTTGTT-3′ | A12 Δsgc; Emr |
| A12-sgc-5′-R-SOE | 5′-CACTCCTTCGAACAGATCCTAATAGCACCGA-3′ | A12 Δsgc; Emr |
| A12-sgc-Erm-F-SOE | 5′-GATCTGTTCGAAGGAGTGATTACATGAACAA-3′ | A12 Δsgc; Emr |
| A12-sgc-Erm-R-SOE | 5′-CAGGATTAGCCCTTTAGTAACGTGTAACTTT-3′ | A12 Δsgc; Emr |
| A12-sgc-3′-F-SOE | 5′-ACTAAAGGGCTAATCCTGGTGCTAGTCAA-3′ | A12 Δsgc; Emr |
| A12-sgc-3′-R | 5′-CCTGGAGAAGAACTATACGTAA-3′ | A12 Δsgc; Emr |
| cipB5′ | 5′-TCATGGATTGAGCTCAAAAAGTAAT-3′ | PcipB::lacZ reporter strain |
| cipB3′ | 5′-TGTATTCATGGATCCAATACCCCTT-3′ | PcipB::lacZ reporter strain |
a
The underlined sequences were included in primers to facilitate PCR ligation of DNA fragments used for mutant construction.