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Heat inactivation of TV at 62, 72, and 80°C, as determined by the PGM-MBs binding assay followed by RT-qPCR. TV was heated at 62°C (A), 72°C (B), or 80°C (C). The receptor binding ability of the heat-treated or untreated virus was determined by the PGM-MBs binding assay, and the RNA from the viral particles retaining receptor binding ability was quantified using RT-qPCR. Each data point represents the mean for three replicates ± 1 standard deviation.
