ECPs of JZ6 and TZ19 cultured at 10°C and 28°C analyzed by SDS-PAGE and Western blotting. Lane M, protein molecular mass standards (in kilodaltons). PJZ6_10, ECP of JZ6 cultured at 10°C; PJZ6_28, ECP of JZ6 cultured at 28°C; PTZ19_10, ECP of TZ19 cultured at 10°C; PTZ19_28, ECP of TZ19 cultured at 28°C. Western blotting detected the extracellular metalloprotease (Vsm) of V. splendidus with polyclonal antiserum (rat anti-Vsm) diluted 1:1,000.