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Scanning electron microscopy (SEM) imaging of B. subtilis 168 spores deposited on glass slides at 300× (1) and 3,000× (2) magnification. (A) Spores (5 × 107) inoculated by liquid spot deposition form multilayered clusters. Spores inoculated by spray deposition with 106 (B), 107 (C), and 5 × 107 (D) spores on sample carriers form homogeneous monolayers. Scale bars represent 50 μm (1) or 5 μm (2).
