TABLE 1.
Inactivation rates of mono- and multilayered B. subtilis spores after treatment with surface decontamination techniques
| Treatment | LD90a |
ICa |
||
|---|---|---|---|---|
| Monolayer | Multilayer | Monolayer | Multilayer | |
| X rays | 140.6 ± 18.4 Gy | 116.2 ± 10.7 Gy | −1.7 × 10−2 ± 1.8 × 10−3 Gy−1 | −1.4 × 10−2 ± 8.1 × 10−4 Gy−1 |
| UV-C | 116.54 ± 4.66 J · m−2* | 695.4 ± 134.20 J · m−2 | −1.6 × 10−2 ± 5.2 × 10−4 J · m−2*** | −3.4 × 10−3 ± 4.5 × 10−4 J · m−2 |
| Blue light | 6.1 × 106 ± 1.7 × 105 J · m−2* | 6.7 × 106 ± 1.1 × 105 J · m−2 | −1.2 × 10−6 ± 1.4 × 10−7 J · m−2 | −1.04 × 10−6 ± 2.3 × 10−8 J · m−2 |
| Argon plasma | 7.4 ± 0.6 s** | 156.1 ± 18.2 s | −1.6 × 10−1 ± 2.8 × 10−3 J · m−2*** | −1.5 × 10−2 ± 1.56 × 10−3 J · m−2 |
a
Shown is a comparison of LD90 values and IC (i.e., slope of semilogarithmic survival curves) of mono- and multilayered B. subtilis 168 spores prepared by spray and liquid deposition, respectively, after treatment with various surface decontamination techniques. Standard deviations and significance are given as determined by ANOVA (*, P < 0.05; **, P < 0.01; ***, P < 0.001).