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. 2016 Mar 2;146(4):673–680. doi: 10.3945/jn.115.222968

FIGURE 1.

FIGURE 1

UHPLC-HRAM trace chromatograms of colonic microbial and phase II metabolites of cocoa flavanols in serum of male pigs fed 0–20 g cocoa powder/d for 27 d. Hydroxybenzoic acid isomers, 3-hydroxyphenylpropionic acid isomers, 3,4-dihydroxyphenylpropionic acid isomers, and O-methyl-epicatechin-glucuronide were detected in serum of pigs fed 0–20 g cocoa powder/d by using UHPLC-HRAM on a Q-Exactive high-resolution mass spectrometer. F, function; FTMS, fourier transform mass spectrometry; NL, normalized level; p ESI SIM, positive mode electrospray ionization selective ion monitoring; RT, retention time; UHPLC-HRAM, ultra-HPLC tandem with high-resolution accurate MS.