Bacterial abundance in large intestine contents of male pigs fed 0–20 g cocoa powder/d for 27 d. Bacterial abundance of intestinal contents from the cecum, proximal colon, and distal colon was measured by real-time PCR with the use of species-specific probes. Lactobacillus casei group (A), Lactobacillus non-casei group (B), Bifidobacterium species (C), and Eubacteria (D) abundance levels are shown. Values are means ± SEMs, n = 6. Labeled means without a common letter differ, P < 0.05.