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. Author manuscript; available in PMC: 2016 Mar 25.
Published in final edited form as: ACS Synth Biol. 2015 Nov 20;5(2):163–171. doi: 10.1021/acssynbio.5b00197

Figure 1.

Figure 1

A genetic code representation that marks the codons decoded by each of the 46 tRNA isoacceptors, whose anticodons and intracellular abundances are stated. In E. coli, the UGA stop codon is reassigned to selenocysteine (U). The decoding patterns and cellular abundances of all 46 endogenous tRNA isoacceptors had previously been reported,26 and we calculated the isoacceptor abundance for each of the 64 codons using this equation: isoacceptor abundance for a codon = (biochemically determined isoacceptor abundance) × (codon usage)/(total codon usage of all codons decoded by isoacceptor). Codon usage values are based on NC_000913.2 (National Center for Biotechnology Information, 1 September 2011) and taken from Lajoie et al.25 N.D. indicates values not determined.