Figure 3.
Size and granularity of adipose-derived stem cells (ASCs) cultured in different medium compositions. (A): Representative example from donor 1 showing the changes in size and granularity between the stromal vascular fraction, passage 1, and passage 7. Size was measured by mean of forward scatter height, and granularity was measured by mean of side scatter height using flow cytometry. The number of cells is presented as the percentage of total number of gated cells. (B): Average size and granularity for all donors reveal an increase in both size and granulation by first passage. The results are presented as mean ± SD. Cell size and granularity were normalized to the cell size and granularity of each individual donor for A-MEMhPL10 at passage 1. ∗∗∗, Statistically different from all other groups at p < .001. #, A-MEMFCS statistically different from all hPL-supplemented media at p < .001. Abbreviations: A-MEM, α-minimum essential medium; FCS, fetal calf serum; FSC, forward scatter; hPL5, 5% human platelet lysate; hPL10, 10% human platelet lysate; P, passage; SSC side scatter; SVF, stromal vascular fraction.