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. 2016 Jan 29;5(3):366–378. doi: 10.5966/sctm.2015-0208

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Figure 2. — Cytotoxicity and apoptosis levels of human mesenchymal stem cells (hMSCs) ejected via 30G needles. (A): Cytotoxicity and apoptosis measurements in hMSCs from 2 donors 4 hours postejection (analyzed by ApoTox-Glo Triplex Assay). Cytotoxicity and caspase-3/7 activity measurements were normalized to viability within the same well. 10 μM staurosporine treatment was used as positive control (n = 5; mean± SD). ∗, p ≤ .05; ∗∗, p < .01; ∗∗∗, p < .0001. (B): Percentages of apoptotic and necrotic cells 24 hours postinjection, measured using Alexa Fluor 488 Annexin V/PI (3 donors in 6 independent experiments; n = 15). ∗, p ≤ .05 for comparison of samples and control using analysis of variance with Dunnett’s post hoc test. Abbreviation: Ctrl, control.