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. 2016 Jan 29;5(3):366–378. doi: 10.5966/sctm.2015-0208

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Figure 3. — Viability and apoptosis levels of human mesenchymal stem cells (hMSCs) delivered via 34G needles. (A): Percentage of hMSCs delivered 24 hours postejection via 34G 20-mm needles. Results are normalized mean values to control ± SD (averages from 2 donors and 2 independent experiments, each n = 5). ∗∗, p ≤ .01 one-way analysis of variance (ANOVA). (B): Representative fluorescence images depicting Live/Dead-stained hMSCs ejected at several flow rates 48 hours postejection. Scale bar = 100 μm. (C): Mean percentages (± SD) of apoptotic and necrotic cells 24 hours postinjection via 34G needles, measured using Alexa Fluor 488 Annexin V/PI (3 donors in 5 independent experiments; n = 11). ∗, p ≤ .05 for comparison of samples and control (ANOVA and Dunnett’s post hoc test). Abbreviation: Ctrl, control.