Fig 1. Soluble YopB is stabilized by YopD.

Samples from cell-free expression of YopB, YopD and YopB/D were native affinity purified from cell-free expression. Lanes represent the following 1) YopD elution; 2) YopD precipitated on affinity beads; 3) YopB elution; 4) YopB precipitated on affinity beads; 5) Elution of YopB co-expressed with YopD; 6) YopB co-expressed with YopD precipitated on affinity beads. Black arrow indicated YopD, Grey arrow indicates YopB. Samples were run on a 4–12% Bis-Tris gel with MES-SDS running buffer and stained with Coomassie brilliant blue. Samples were run on a 4–12% Bis-Tris gel with MES-SDS running buffer and stained with Coomassie brilliant blue, followed by destaining and imaging. The molecular weight markers are SeeBlue Plus2 prestained markers from Life Technologies.