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. 2016 Mar 25;11(3):e0150166. doi: 10.1371/journal.pone.0150166

Fig 2. Co-expression of YopB, YopD, or YopB/D with NLP-forming Δ49ApoA1 increases the solubility of these proteins.

Fig 2

SDS-PAGE of cell-free expressed A) YopB B) YopD and C) YopB/D. Lanes correspond to expression experiments (Lanes 1–3) in the absence of lipids; (lanes 4–6) in the presence of DMPC lipid vesicles; (Lanes 7–9) and in the presence of DMPC lipids as well as the co-expression of Δ49ApoA1. Crude reactions were separated into total (T), soluble (S) and pellet (I) fractions, separated by a 4–12% Bis-Tris SDS-PAGE gel with MES-SDS running buffer. Proteins were detected by fluorescence of an incorporated Fluortect-green (Promega) labeled lysine added to the cell-free reactions. Grey arrow YopB; Black arrow YopD; White arrow Δ49ApoA1.