Fig 5. Diffusion curves of protein, empty NLPs, protein associated NLPs, and liposomes measured by FCS.

The diffusion curves for YopD (blue) and the DMPC liposomes (orange) are derived from the autocorrelation of their corresponding fluorescence (YopD: bodipy, DMPC: Texas red) intensity time traces, while the diffusion curve for empty NLPs (black) is derived from the cross correlation of bodipy signal (labeled on Δ49ApoA1) and the Texas red signal (labeled on DMPC lipids) of their corresponding fluorescence intensity time traces, and the diffusion curves for YopD-NLPs (purple) and YopB/D-NLPs (green) are derived from the cross correlation of the GFP signal (labeled on Yop proteins) and the Texas red signal (labeled on DMPC lipids) of their corresponding fluorescence intensity time traces.