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. 2016 Mar 25;11(3):e0150166. doi: 10.1371/journal.pone.0150166

Fig 6. Anti-YopD western blot confirms YopD complex formation with NLPs.

Fig 6

Purified expressed YopB and/or YopD co-expressed with Δ49ApoA1 in the presence of lipid. A) Native 4–20% Tris-glycine PAGE gel, Native Tris-glycine buffer, detection by SyproRuby fluorescence total protein stain. B) Anti-YopD western blot of a native 4–20% Tris-glycine PAGE gel, Native Tris-glycine buffer, detection by fluorescence of rhodamine conjugated secondary antibody. The lanes are represented as follows: 1) YopB with Δ49ApoA1; 2) YopB/D with Δ49ApoA1; 3) YopD with Δ49ApoA1; 4) Δ49ApoA1 only (empty-NLP); NM) Protein mass standard (kDa) NativeMark (Invitrogen).