Table 1. Percent of total Yop protein in the soluble fraction.
Reactions expressing YopB | Reactions expressing YopD | ||||
Reaction | % soluble | stdev | Reaction | % soluble | stdev |
YopB | 14.1 | 4.5 | YopD | 33.4 | 20.3 |
YopB + lipid | 15.2 | 4.7 | YopD + Lipid | 47.1 | 33.2 |
YopB-NLP | 44 | 17.6 | YopD-NLP | 58.7 | 15.3 |
Reactions expressing both YopB and YopD | |||||
YopB Bands | % soluble | stdev | YopD Bands | % soluble | stdev |
YopB/D | 8.6 | 1.5 | YopB/D | 13.5 | 0.6 |
YopB/D + Lipids | 17.6 | 8 | YopB/D + Lipids | 17.1 | 6.9 |
YopB/D-NLP | 28.3 | 8.8 | YopB/D-NLP | 41 | 8.9 |