Table 2. Size distribution and membrane protein incorporation rate of co-expressed YopB- and/or YopD-NLPs as determined by AFM.
| Sample | Populations observed | Height (nm) | Diameter (nm) | Incorporation rate |
|---|---|---|---|---|
| Empty-NLPs | A: empty-NLPs only | 4.6 ± 0.3 | 13.6 ±1.7 | N/A |
| YopB-NLPs | B.1: empty-NLPs | 4.6 ± 0.3 | 12.1 ± 2.1 | --- |
| B.2: YopB-NLPs | 5.8 ± 0.3* | 14.0 ± 3.1 | 30% | |
| YopB/D-NLPs | C.1: empty-NLPs | 4.9 ± 0.3 | 14.5 ± 3.0 | --- |
| C.2:YopB/D-NLPs | 6.1 ± 0.3* | 18.9 ± 4.8 | 39% | |
| YopD-NLPs | D: empty- and YopD-NLPs | 4.8 ± 0.3 | 14.9 ± 3.0 | indeterminate |
*p-values<0.001