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. 2016 Mar 25;12(3):e1005513. doi: 10.1371/journal.ppat.1005513

Fig 1. BgTLR displayed increased transcript expression in BS-90 snails following challenge with S. mansoni.

Fig 1

(A) BgTLR transcript expression with and without S. mansoni challenge. Snails (BS-90 and M-line strains) were individually exposed to ~5 miracidia or left unexposed (control). Five snails were collected at indicated time points over the incubation period of the parasite. RNA was extracted from whole snails, converted to cDNA and BgTLR expression was measured by quantitative PCR. Expression was quantified in fold changes normalized to time 0-hour controls. Bars represent standard error (n = 5). Asterisk (*) indicates significant difference (P < 0.05) between experimental and control samples, while hash (#) indicates significant difference between BS-90 and M-line snails at the respective time points. (B) Knockdown of BgTLR or GFP (control) in BS-90 snails responding to S. mansoni parasite challenge. (C) Knockdown of BgTLR in unexposed M-line and BS-90 snails. Five snails were collected for each time point in B and C for RNA extraction and cDNA synthesis. Bars represent standard error (n = 5). Asterisks indicate significant difference (P < 0.05) from 0-hour time points.