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. 2016 Mar 25;11(3):e0152129. doi: 10.1371/journal.pone.0152129

Fig 3. Decreased Proliferation and Adipogenic Gene Profile of Stromal Vascular Fraction in Mice with Slow Muscle Fiber-Type Predominance.

Fig 3

(A) Percentage of BrdU-positive (BrdU+) cells within total stromal vascular fraction (SVF) of wild-type (Wt) and miR-499 Tg subcutaneous depots. BrdU was given as a two-hour pulse injected intraperitoneally (IP). (B) Percentage of BrdU+ cells within total SVF of Wt and Sox6 mKO subcutaneous depots. BrdU was given as a two-hour pulse injected IP. (C) Oil Red O staining of plated subcutaneous SVF after four days of insulin induction of both Wt and mir-499 Tg mice. (D) qPCR of adipogenic markers from adipogenically-induced SV cells isolated from subcutaneous adipose depots from Wt and mir-499 Tg mice. (E) Oil Red O staining of plated subcutaneous SVF after four days of insulin induction of both Wt and Sox6 mKO mice. (F) qPCR of adipogenic markers from adipogenically-induced SVF isolated from subcutaneous adipose depots from Wt and Sox6 mKO mice. Representative studies on P60 females; n ≥ 8 per cohort, repeated ≥ 3 cohorts. Error bars indicate SEM. Statistical significance assessed by two-tailed Student’s t-test. *p<0.05, **p<0.01.