Fig 2. BC-23 inhibits Wnt/β-catenin signaling.
H1299 cells were co-transfected with the reporter TOP-flash and the control Renilla plasmids. Four hours after transfection, cells were treated with various concentrations of BC-23 for 24 h. The TOP luciferase activities were determined and normalized to Renilla luciferase activities. BC-23 exhibited dose-dependent inhibitory activity on TOP luciferase in H1299 cells, with an IC50 value of 2.3 μM. The columns represent mean ± S.D. of 3 independent experiments.