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. 2016 Mar 25;11(3):e0152407. doi: 10.1371/journal.pone.0152407

Fig 5. ROS generation is responsible for BC-23 induced radiation enhancement and clonogenic cell death.

Fig 5

A. Combining 3 μM BC-23 with 10 Gy radiation (x) dramatically increased ROS generation, when compared to individual treatments alone: vehicle control (●), 3 μM BC-23 (■), and 10 Gy radiation (▲). Each value is the mean ± SD of three separate experiments. B-D, ROS-dependent cytotoxic effects of BC-23. NAC attenuates the cell death mediated by exposure of H1299 cells to the indicated concentrations of BC-23 in cytotoxicity (B) and colongenic assays (C, representative dishes of clonogenic assays and D, analyses of percentage of surviving cells). *, p<0.05 versus control.