Fig 5. Wild type and mutant FGFR1 reduced neurite length on growth permissive PLL and growth inhibitory CSPGs when compared to control cells.

A) Schematic diagram of the pMaxGFP vector highlighting distinct features. CMV: cytomegalovirus. B) Representative pictures of cerebellar granule neurons grown on CSPG. C,D) Cells were transfected with 1μg pMaxGFP and 4μg of either wild type or mutant FGFR1 by electroporation and then cultured on PLL (C) or CSPGs (D) for 48 hours. Control cells were transfected with 1μg pMaxGFP. Cells were stained for βIII tubulin and DAPI and analysed for neurite outgrowth by fluorescence microscopy. Data are mean ± SEM (n = 8) and significance is shown for Dunnett’s post hoc test values when groups were compared to cells transfected with pMaxGFP alone. *P < 0.05; **P < 0.01; ***P < 0.001; ns–not significant.