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. 2016 Mar 25;11(3):e0151876. doi: 10.1371/journal.pone.0151876

Table 1. Ingredients proportion and chemical composition of the experimental diets.

Item Inclusion of canola oil
Control 3% 6%
Ingredient g/kg of DM
Ground corn 297.30 261.40 225.30
46%CP Soybean meal 174.70 180.60 186.70
NaCl 5.00 4.90 4.90
Mineral mixture1 13.80 13.80 13.80
Dicalcium phosphate 1.10 1.40 1.40
Urea 5.00 4.90 4.90
Limestone 2.30 2.40 2.40
Canola oil - 30.00 60.00
Maize silage 500.20 500.00 500.00
Chemical composition, g/kg of DM
DM 638.50 638.50 641.30
MM 39.00 38.80 38.60
OM 960.09 961.10 961.30
NDF 299.00 296.30 293.70
ADF 184.20 183.50 182.80
iADF 69.00 68.70 68.30
ADL 35.90 35.80 35.60
EE 31.90 60.10 88.20
CP 170.50 170.20 169.90
TC2 758.40 730.80 703.10
NFC3 459.30 434.40 409.40
TDN4 669.30 691.40 727.80
NEl, Mcal/Kg5 15.20 15.70 16.60

1Mineral mixture composition per kilogram: 242 g of Ca [minimum (min)], 30 mg of Co (min), 1,008 mg of Cu (min), 80 g of S (min), 390 mg of Fl (max), 39 g of P (min), 60 mg of I (min), 20 g of Mg (min), 2,998 mg of Mn (min), 1,100 mg of monensin sodium (min), 30 mg of Se (min), 4,032 mg of Zn (min), 400,000 IU of vitamin A (min), 40,000 IU of vitamin D3 (min), and 1,450 IU of vitamin E (min). MM = mineral matter; OM = organic matter; NDF = neutral detergent fiber ADF = acid detergent fiber; iADF = indigestible ADF; ADL = acid detergent lignin; EE = ether extract; CP = crude protein

2TC = total carbohydrates [12]

3NFC = no fibrous carbohydrates [13]

4TDN = total digestible nutrients [12]

5NEl = net energy for lactation [11].