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. Author manuscript; available in PMC: 2016 Mar 25.
Published in final edited form as: J Nanomed Nanotechnol. 2015 Dec 27;6(6):340. doi: 10.4172/2157-7439.1000340

Figure 2.

Figure 2

ESAT-6 increases fibrosis. Figures show representative sections of lung from each treatment or control group (Trichrome, 200×) (n=3/group). No fibrotic changes were present in either sham-treated controls (A) or mice receiving ESAT-6 alone (B). Mice instilled with MWCNT alone exhibited little fibrosis in granuloma and non-granuloma areas (C). The combination of MWCNT + ESAT-6, however elicited marked fibrosis: the trichrome stain highlights the presence of collagen fibers (stained blue) associated with granulomas (D). qPCR analysis of lung tissues obtained by LCM revealed significant (p ≤ 0.05) elevation of fibronectin (Fn1) in granuloma (+) tissue from mice instilled with MWCNT + ESAT-6 compared to granuloma (-) tissue (E).