Skip to main content
. 2016 Mar 21;9:1697–1706. doi: 10.2147/OTT.S97189

Figure 3.

Figure 3

Knocking down the expression of PLCB1 inhibits the growth and increases apoptosis of Hep3B and LM3 cells.

Notes: (A) Knocking down the expression of PLCB1 in Hep3B and LM3 was confirmed via Western blot. (B) Cell viability of Hep3B and LM3 cells with PLCB1 knocking down was measured by CCK-8. Data were representative as the mean ± SEM of triplicated independent experiments. (C) The effects of PLCB1 on the growth of Hep3B and LM3 cells were measured by colony formation assay. Results were described as the relative number from five randomly selected fields. The data were representative as the mean ± SEM of triplicated independent experiments. (D) Caspase-3/7 activity of Hep3B and LM3 cells after knocking down PLCB1 was detected. The data were representative as the mean ± SEM of triplicated independent experiments (*represents sh-1 compared to shcon, #represents sh-2 compared to shcon, *P<0.05, **P<0.01, #P<0.05, ##P<0.01).

Abbreviations: PLCB1, phospholipase C, β1; CCK, cell counting kit; shRNAs, short hairpin RNAs; sh-1 and sh-2, lentivirus-delivered shRNAs; shcon, control shRNA; h, hours; SEM, standard error of the mean.