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A., B. The expression of E2F1 was examined by Western blot analysis in HepG2 and H7402 (or HepG2.2.15) cells transfected with pcDNA3.1-HULC (or si-HULC-1). The transfection efficiency of HULC (or si-HULC-1) was detected by qRT-PCR. C. The correlation between E2F1 mRNA levels and HULC mRNA levels was detected by qRT-PCR in 60 HCC tissues (P < 0.001, r = 0.962, Pearson's correlation). *P < 0.05; **P < 0.01; Student's t test.
